Bgl II
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Apa I
Bal31 Nuclease
Hha I
λ/Hind III digest-ΦX174/Hinc II digest
rTaq DNA Polymerase(不含Mg2+)用Buffer
λ/EcoR I digest-λ/Hind III digest
Pac I
KOD-Plus- Mutagenesis Kit
L Buffer