Syntenin 1,多配体聚糖结合蛋白1抗体

产品编号HZ-11379R
英文名称Syntenin 1
中文名称多配体聚糖结合蛋白1抗体
别 名Sdcbp; MDA-9; MDA9; Melanoma differentiation-associated protein 9; Pro-TGF-alpha cytoplasmic domain-interacting protein 18; Scaffold protein Pbp1; SDCB1_HUMAN; Sdcbp; ST1; SYCL; Syndecan binding protein 1; Syndecan-binding protein 1; Syntenin 1; Syntenin-1; TACIP18.
说 明 书0.1ml 0.2ml
研究领域细胞生物 神经生物学 细胞类型标志物
抗体来源Rabbit
克隆类型Polyclonal
交叉反应 Human, Mouse, Rat, Chicken, Dog, Rabbit,
Syntenin 1,多配体聚糖结合蛋白1抗体产品应用WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量32kDa
细胞定位细胞核 细胞浆 细胞膜
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human Syntenin 1
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Syntenin 1,多配体聚糖结合蛋白1抗体PubMedPubMed
产品介绍background:
Syntenin-1 (also known as syntenin, syndecan binding protein, melanoma differentiation-associated protein 9 or proTGF-alpha cytoplasmic domain-interacting protein 18) is a protein that binds to the cytoplasmic domains of the syndecans in yeast 2-hybrid screens and other assays. Syntenin-1 contains a tandem repeat of PDZ domains that reacts with the FYA (phe-tyr-ala) carboxy-terminal amino acid sequence of the syndecans. It may function as an adaptor that couples syndecans to cytoskeletal proteins or cytosolic downstream signal-effectors. Syntenin-1 colocalizes and interacts specifically with immature, intracellular forms of proTGF-alpha. It is a human gamma interferon responsive protein. Syntenin-1 contains PSD-95/Discs large/zO-1 (PDZ) domains and associates with the cytoplasmic tail of the IL-5Ralpha. It directly associates with the transcription factor Sox-4. The PDZ proteins PICK1, GRIP, ABP and syntenin-1 bind multiple glutamate receptor subtypes.

Function:
Seems to function as an adapter protein. In adherens junctions may function to couple syndecans to cytoskeletal proteins or signaling components. Seems to couple transcription factor SOX4 to the IL-5 receptor (IL5RA). May also play a role in vesicular trafficking. Seems to be required for the targeting of TGFA to the cell surface in the early secretory pathway.

Subunit:
Monomer and homodimer. Interacts with SDC1, SDC2, SDC3, SDC4, NRXN2, EPHA7, EPHB1, NF2 isoform 1, TGFA and IL5RA. Interacts with neurofascin, SDCBP2 and PTPRJ.

Subcellular Location:
Cell junction, focal adhesion. Cell junction, adherens junction. Cell membrane; Peripheral membrane protein. Endoplasmic reticulum membrane; Peripheral membrane protein. Nucleus. Melanosome. Cytoplasm, cytosol. Cytoplasm, cytoskeleton. Note=Mainly membrane-associated. Localized to adherens junctions, focal adhesions and endoplasmic reticulum. Colocalized with actin stress fibers. Also found in the nucleus. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.

Syntenin 1,多配体聚糖结合蛋白1抗体Tissue Specificity:
Widely expressed. Expressed in fetal kidney, liver, lung and brain. In adult highest expression in heart and placenta.

Post-translational modifications:
Phosphorylated on tyrosine residues.

Similarity:
Contains 2 PDZ (DHR) domains.

Gene ID:
6386

Database links:
Entrez Gene: 510979 Cow
Entrez Gene: 6386 Human
Entrez Gene: 53378 Mouse
Entrez Gene: 83841 Rat
Omim: 602217 Human
SwissProt: O00560 Human
SwissProt: O08992 Mouse
SwissProt: Q9JI92 Rat
Unigene: 200804 Human
Unigene: 247473 Mouse
Unigene: 483278 Mouse
Unigene: 4309 Rat

Syntenin 1,多配体聚糖结合蛋白1抗体Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品图片

Tissue/cell: Mouse placenta tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;  Incubation: Anti-Syntenin 1 Polyclonal Antibody, Unconjugated(HZ-11379R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Tissue/cell: Mouse Embryo tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;  Incubation: Anti-Syntenin 1 Polyclonal Antibody, Unconjugated(HZ-11379R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining