PDPK1 polyclonal antibody
产品名称： PDPK1 polyclonal antibody
英文名称： PDPK1 polyclonal antibody
产品编号： PAB4570
产品价格： null
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围：
亚诺法生技股份有限公司（Abnova)
- 联系人 :
- 地址 : 台湾台北市内湖区洲子街 108 号 9 楼
- 邮编 : 11493
- 所在区域 : 台湾
- 电话 : +886-920**1152 点击查看
- 传真 : 点击查看
- 邮箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Rabbit polyclonal antibody raised against synthetic peptide of PDPK1.
- Immunogen:
- A synthetic peptide (conjugated with KLH) corresponding to N-terminus of human PDPK1.
- Host:
- Rabbit
- Reactivity:
- Human
- Form:
- Liquid
- Purification:
- Protein G purification
- Storage Buffer:
- In PBS (0.09% sodium azide)
- Storage Instruction:
- Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.
- Recommend Usage:
- ELISA (1:1000)
Western Blot (1:250-500)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.
- Note:
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Publication Reference
- 1.
- Peroxisomal targeting as a tool for assaying potein-protein interactions in the living cell: cytokine-independent survival kinase (CISK) binds PDK-1 in vivo in a phosphorylation-dependent manner.
Nilsen T, Slagsvold T, Skjerpen CS, Brech A, Stenmark H, Olsnes S.J Biol Chem. 2004 Feb 6;279(6):4794-801. Epub 2003 Nov 6.
- 2.
- In vivo role of the PIF-binding docking site of PDK1 defined by knock-in mutation.
Collins BJ, Deak M, Arthur JS, Armit LJ, Alessi DR.EMBO J. 2003 Aug 15;22(16):4202-11.
- 3.
- Membrane localization of 3-phosphoinositide-dependent protein kinase-1 stimulates activities of Akt and atypical protein kinase C but does not stimulate glucose transport and glycogen synthesis in 3T3-L1 adipocytes.
Egawa K, Maegawa H, Shi K, Nakamura T, Obata T, Yoshizaki T, Morino K, Shimizu S, Nishio Y, Suzuki E, Kashiwagi A.J Biol Chem. 2002 Oct 11;277(41):38863-9. Epub 2002 Jul 29.
- Applications
- Western Blot (Cell lysate)
- Western blot analysis of PDPK1 polyclonal antibody (Cat # PAB4570) in HepG2 cell line lysates . PDPK1 Antibody (N-term) (arrow) was detected using the purified PDPK1 polyclonal antibody (Cat # PAB4570) .
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Formalin-fixed and paraffin-embedded human breast carcinoma reacted with PDPK1 polyclonal antibody (Cat # PAB4570) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining . This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated .
- Interactome
- Related Disease