Introduction
Nitric oxide (NO) was initially identified as a muscle relaxation factor, mediating its effects on smooth
muscle by coordinating the heme moiety of guanylate cyclase, leading to enhanced production of
cGMP. Besides smooth muscle relaxation, NO also influences a variety of biological systems
including cell proliferation, apoptosis, neurotoxicity, Parkinson disease, extracellular matrix
remodeling. Many of its effects are mediated through protein nitration.
Peroxynitrite (ONOO–) is formed in the chemical reaction between nitric oxide and superoxide.
ONOO– nitrates free tyrosine and tyrosine residues in proteins; modifications that are used as markers
of ONOO– action under conditions of cellular damage and in numerous diseases.
Cell Biolabs’ Nitrotyrosine Immunoblot Kit offers a simple and complete system for the detection of
nitrotyrosine in proteins. This kit also includes Nitrated BSA as positive control. Each kit provides
sufficient quantities to perform at least 10 blots (7.5 cm x 8.5 cm).