PPT1 (C-term) Peptide
产品名称： PPT1 (C-term) Peptide
英文名称： PPT1 (C-term) Peptide
产品编号： P1156
产品价格： 0
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围： null
亚诺法生技股份有限公司（Abnova)
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- 所在区域 : 台湾
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- 邮箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Rabbit polyclonal antibody raised against synthetic peptide of PPT1.
- Immunogen:
- A synthetic peptide (conjugated with KLH) corresponding to C-terminus of human PPT1.
- Host:
- Rabbit
- Reactivity:
- Human
- Form:
- Liquid
- Purification:
- Protein G purification
- Storage Buffer:
- In PBS (0.09% sodium azide)
- Storage Instruction:
- Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.
- Recommend Usage:
- ELISA (1:1000)
Western Blot (1:100-500)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.
- Note:
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Publication Reference
- 1.
- The crystal structure of palmitoyl protein thioesterase-2 (PPT2) reveals the basis for divergent substrate specificities of the two lysosomal thioesterases, PPT1 and PPT2.
Calero G, Gupta P, Nonato MC, Tandel S, Biehl ER, Hofmann SL, Clardy J.J Biol Chem. 2003 Sep 26;278(39):37957-64. Epub 2003 Jul 10.
- 2.
- Neuronal ceroid lipofuscinoses caused by defects in soluble lysosomal enzymes (CLN1 and CLN2).
Hofmann SL, Atashband A, Cho SK, Das AK, Gupta P, Lu JY.Curr Mol Med. 2002 Aug;2(5):423-37.
- 3.
- The neuronal ceroid lipofuscinoses: mutations in different proteins result in similar disease.
Weimer JM, Kriscenski-Perry E, Elshatory Y, Pearce DA.Neuromolecular Med. 2002;1(2):111-24.
- Applications
- Western Blot (Cell lysate)
- The PPT1 polyclonal antibody (Cat # PAB2508) is used in Western blot to detect PPT1 in 293 cell lysate.
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with the PPT1 polyclonal antibody (Cat # PAB2508) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. HC = hepatocarcinoma.
- Entrez GeneID:
- 5538
- Protein Accession#:
- PPT1_HUMAN
- Gene Name:
- PPT1
- Gene Alias:
- CLN1,INCL,PPT
- Gene Description:
- palmitoyl-protein thioesterase 1
- Gene Ontology:
- Hyperlink
- Gene Summary:
- The protein encoded by this gene is a small glycoprotein involved in the catabolism of lipid-modified proteins during lysosomal degradation. The encoded enzyme removes thioester-linked fatty acyl groups such as palmitate from cysteine residues. Defects in this gene are a cause of infantile neuronal ceroid lipofuscinosis 1 (CLN1, or INCL) and neuronal ceroid lipofuscinosis 4 (CLN4). Two transcript variants encoding different isoforms have been found for this gene
- Other Designations:
- OTTHUMP00000004836,ceroid-palmitoyl-palmitoyl-protein thioesterase 1,palmitoyl-protein hydrolase 1
- Related Disease