来自吉林大学第一医院和美国埃默里大学温希普癌症研究所的科研人员合作发现了一个Dicer调控细胞周期的新功能*，这是一个意想不到的实验发现，相关成果提前发表在1月的Nucleic Acids Research上。此项目中的miRNA芯片检测由联川生物承担完成。

DICER-dependent biogenesis of let-7 miRNAs affects human cell response to DNA damage via targeting p21/p27

Recently, it was reported that knockdown of DICER reduced the ATM-dependent DNA damage response and homologous recombination repair (HRR) via decreasing DICER-generated small RNAs at the damage sites. However, we found that knockdown of DICER dramatically increased cell resistance to camptothecin that induced damage required ATM to facilitate HRR. This phenotype is due to a prolonged G1/S transition via decreasing DICER-dependent biogenesis of miRNA let-7, which increased the p21Waf1/Cip1/p27Kip1 levels and resulted in decreasing the HRR efficiency. These results uncover a novel function of DICER in regulating the cell cycle through miRNA biogenesis, thus affecting cell response to DNA damage.

    最近，有报道，敲减Dicer会降低ATM依赖性的DNA损伤应答和同源重组修复（HRR），是由于在损伤位点处Dicer产生的小RNA减少了。然而，我们发现敲减Dicer会显著增加细胞对喜树碱的抗性，喜树碱诱导的损伤需要ATM来促进HRR。这种表型是由于降低了Dicer依赖性的miRNA let-7的生物合成，该过程会提高p21WAF1/CIP1/p27kip1水平，进而延长了G1/S过渡期，并会导致HRR效率下降。这些结果揭示出一个Dicer调控细胞周期的新功能，是通过控制miRNA生物合成，从而影响细胞对DNA损伤的应答。

*Liu B, Liu M, Wang J, Zhang X, Wang X, Wang P, Wang H, Li W, Wang Y. (2015) DICER-dependent biogenesis of let-7 miRNAs affects human cell response to DNA damage via targeting p21/p27. Nucleic Acids Research. [Epub ahead of print]

联川生物客户在《自然》发现调控肿瘤生长的开关！

获取更多用户研究案例，请访问www.lc-bio.com或拨打热线800-857-1452。